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In order to characterize variation introduced by deconvolution, we generated output from five different deconvolution pathways for the same input data, directing these outputs to msAlign format for identification by TopPIC. Bruker maXis data were processed by all pipelines except for ThermoFisher Xtract, and ThermoFisher Orbitrap data were processed by all pipelines except for Bruker DataAnalysis.

Journal: Journal of Proteome Research

Article Title: Comparing Top-Down Proteoform Identification: Deconvolution, PrSM Overlap, and PTM Detection

doi: 10.1021/acs.jproteome.2c00673

Figure Lengend Snippet: In order to characterize variation introduced by deconvolution, we generated output from five different deconvolution pathways for the same input data, directing these outputs to msAlign format for identification by TopPIC. Bruker maXis data were processed by all pipelines except for ThermoFisher Xtract, and ThermoFisher Orbitrap data were processed by all pipelines except for Bruker DataAnalysis.

Article Snippet: A Visual Basic Script for Bruker DataAnalysis (described below) performed feature detection from the raw Q-TOF data, deconvolved MS/MS peak lists, and exported msAlign files.

Techniques: Generated